Part:BBa_K4165144
MINK Peptide
A synthetic peptide that is used for targeting misfolded tau protein (BBa_K4165009) as it binds to PHF6 of tau fibrils.
Usage and Biology
MINK is a peptide with amino acid sequence of DVQMINKKRK, it is acquired by mirror phage display to inhibit the fibrillization of tau which is one of the main drivers of Alzheimer’s disease and other dementia types. PHF* (VQIINK) is one of the sites that derives tau aggregation. MINK can bind to PHF* in a way that can disrupt the interaction between the fibrils and consequently reduce the aggregates. Its IC50 for inhibition of tau aggregates were 22.6 μM.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Dry Lab
Modeling
MINK was modeled by AlphaFold2, Apptest, ITAsser and TrRosetta, and the best model obtained from Apptest ranking 6 out of 6
Figure 1.: Predicted 3D structure of Synthetic peptide MINK.
Table 1: Quality assessment parameters of MINK model.
References
1. Seidler, P. M., Boyer, D. R., Rodriguez, J. A., Sawaya, M. R., Cascio, D., Murray, K., ... & Eisenberg, D. S. (2018). Structure-based inhibitors of tau aggregation. Nature chemistry, 10(2), 170-176.
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